A stability-indicating tablet method is not automatically a validated forensic biological-fluid method.
The laboratory comparison developed screen-printed and solid-contact potentiometric electrodes for bromazepam. Response depended on pH, with useful performance in an acidic range. The investigators tested bromazepam in the presence of a major degradation product and applied the methods to tablets.
The selected interferent challenge does not establish selectivity against every benzodiazepine, metabolite or endogenous matrix component. Calibration in controlled solutions and tablets cannot independently validate blood, urine or postmortem samples. Electrochemical response also requires a matrix-appropriate measurement procedure.
For an analytical opinion, distinguish the intended pharmaceutical-quality question from drug identification in a person. Ask which degradation products and interferents were actually tested. The study supports an assay-development result without establishing a universal screening or confirmation method for forensic casework.
Source reviewed: A Comparative Study between Screen Printed and Solid Contact Electrodes for the Stability Indicating (PMC9659083). Forensic applications above are interpretive analysis of the supplied publication.
Related reading: Remimazolam and Malignant-Hyperthermia Mutations: A Cell Assay Is Not a Patient Safety Trial.