A laboratory can measure several drugs in a single chromatographic run. Each drug still needs a calibration range that covers the concentrations the assay is intended to report.
Hu and colleagues validated a UPLC–MS/MS assay for celecoxib, dezocine and dexmedetomidine in beagle plasma. Their calibration ranges were 10–2,000, 1–200 and 0.1–20 ng/mL, respectively. The drugs were given together by different routes in the pharmacokinetic study. The researchers evaluated selectivity, accuracy, precision, recovery and stability as part of the method validation.
Read the calibration and quality-control results for the analyte in question. A concentration within the validated range for celecoxib can be outside the range for dexmedetomidine in the very same tube. If an analyte exceeds its upper limit, the report should identify the validated procedure used to bring it into range, if one was applied. The study’s validation applies to beagle plasma under the conditions tested. Other matrices require their own supporting data.
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