The study compared high-performance liquid chromatography with an ultra-high-performance implementation for seven benzodiazepines. The reported run time fell from 40 to 15 minutes. The systems also used different column particle sizes, operating pressures, flow rates and injection volumes.
The authors reported lower detection limits for all seven targets under the faster conditions. They calculated detection limits using a specified signal-to-noise rule. A detection limit established in that experiment is a different quantity from the lowest concentration another laboratory can report quantitatively in a particular specimen.
Identify the detector, extraction, specimen matrix, target list and applicable reporting criteria. Then review any evidence of calibration and precision for the analyte at issue. This study illustrates how a method transfer can change performance, while leaving the performance of another laboratory’s panel to that laboratory’s own data.